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Product Introduction

Sofast® Transfection Reagent is a new generation cationic polymer gene transfectionreagent. Comparedwith cationic lipids, cationic polymers are very stable, easy to handle and more resistant toserum in cell culture. The above advantages make transfection much easier and eproducible. Sofast®are widely used for both primary cell and established cell lines.

Product: Sofast® Transfection Reagent


Use: For research use only. Not for human or drug use.

Storage: Store at 4℃,Don't freeze.

   ◆ High transfection efficiency:  Sofast® shows high transfection efficiency,especially higher transfection efficiency in HUV-EC(primary cell) compared with cationic lipid. Some of ransfection efficiency with Sofast®: HEK293(95%)、COS7(85%)、HeLa(85%)、HepG2(40~60)、HUV-EC(20~35%);

   ◆ Low cytoxicity: The cell survival rate is over 90%,when experiment is carried in suitable condition;

  Simplified transfection procedure: Sofast® is a serum resistant reagent,the transfection procedure is very simple: the DNA/transfection reagent complexes can be directly added into complete cell medium and waited for transfection  assay,without the worry about changing medium.

Sofast ® Successfully transfected partial cell lines

Cell Line
Source
Cell Type
HEK 293HumanEmbryonic kidney
HeLaHumanCervix carcinoma
NIH 3T3MouseEmbryo fibroblast
BNL CL2MouseEmbryonic kidney cells
HepG2HumanHepatocarcinoma
COS7MonkeySV40 liver transformed
CHOChinese hamsterOvary
SHSY-5YHumanNeuroblastoma
IMR 32HumanNeuroblastoma
MRC5HumanFetal lung epithelium
MCF7HumanBreast adenocarcinoma
K562HumanChronic leukemia
SKOV-3HumanOvarian adenocarcinoma
IGROV-1HumanOvarian adenocarcinoma
HUV-EC(primary cell)HuamnUmbilical vein endothelial cell
C6RatGlioma C6 cell
Primary cellRatNeonatal Rat Cardiomyocyte
SGC-7901HumanGastric cancer )
Eca-109HumanEsophageal carcinoma
SK-N-SHHumanNeuroblastoma
ZR-75-1HumanBreast cancer
THP-1HumanMonocyte
WEHI231MouseImmature B
SK-HEP-1HumanLiver adenocarcinoma
P815MouseMastocytoma
BGC-823HumanGastric carcinoma
CV-1African green monkeyKidney
SiHaHumanCervical carcinoma
A549HumanLung carcinoma
MDA-MB-231HumanBreast adenocarcinoma
T-47DHumanBreast cancer
U-937HumanHistiocytic lymphoma
HuH-7HumanHepatoma
C929HumanBbone marrow
XG-1HumanMyeloma
MC3T3-E1RatOsteogenic
Hep-2HumanLarynx carcinoma squamou
MDCKDogKidney (Madin-Darby)
BMSCMouseBone marrow stromal
BEL-7402HumanHhepatic carcinoma
2BSHumanDiploid fibroblast
U373HumanGlioblastoma astrocytoma

Price Listing:



ProductsCat. No.SizeNote
Sofast® Transfection Reagent111030.3 ml50-100 Transfections/35 dish
Sofast® Transfection Reagent111050.5 ml80-165 Transfections/35 dish
Sofast® Transfection Reagent111104×0.25 ml165-330 Transfections/35 dish
Sofast® Transfection Reagent111202×1.0 ml330-660 Transfections/35 dish
Sofast® Transfection Reagent111404×1.0 ml660-1320 Transfections/35 dish

Download:

Protocol in vivo.pdf